關於RNA polymerase,何者為是?
1. Require a primer to initiate synthesis. RNA polymerase不需要primer
2. Require all four deoxynucleotide triphosphates(dNTPs).
3. With 3’ to 5’ exonuclease activity. mRNA的校正是一種自然發生的水解
4. Synthesis RNA in a 5’ to 3’ direction.
Ans. 4
Which of the following statements is not true about the 5’-end cap structure of mature eukaryotic mRNAs?
1. The cap is methylated.
2. The cap protects the mRNA from degradation.
3. The cap is a residue of 7-methyluridine. (7-Methylguanine)
4. It participates in the binding of the mRNA to the ribosome to initiate translation.
Ans. 2
1. Which of the following is not a form of RNA processing of eukaryotic RNA polymerase 2 primary transcrips ?
(1). Polyadenylation at 3'end
(2). Proteolytic cleavage
(沒有蛋白質的裂解)
(3). Splicing
(4). 5'-end capping
Ans:(3)
1.何者不含有Intron?
mRNA
2.何者屬於Coding RNA?
3.何者負責RNA的轉錄? RNA的序列與何者相同?
RNA polymerase/Coding strand
4. 在原核細胞中,高度保留供轉錄起始識別的序列常位於哪兩個位置?
-10region TATAAT
-35region TTGACA
5. DNA序列上,+1代表?
mRNA的起始點也是轉錄的起始點(TSS)
6. 原核細胞中,何者協助RNA polymerase辨識啟動子(Promoter)?
σ因子
7. 真核細胞中與σ因子具有相似功能的蛋白稱為?
TATA Box-Binding Protein
8.DNA上特定non-coding保留序列對基因轉錄有活化的效果者稱為?
Enhancer
9.透過連接Enhancer對Transctiption factor加強其作用之蛋白稱為?
Activator
10. TAF1於轉錄起始中的作用為何?
乙醯化Histone
將鏈結用的H1標記以降解
11.關於RNA polymerase,何者為是?
a. require a primer to initiate synthesis.
b. require all four deoxynucleotide triphodphates (dNTPs).
c. With 3’ to 5’ exonuclease activity.
d. synthesize RNA in a 5’ to 3’ direction.
12.Rho(p)因子主要參與轉錄哪一階段?
終止RNA轉錄
13.何種序列與Poly A tail 的形成有關?
AAUAAA
14.關於真核細胞mRNA,何者錯誤?(Which of the following statements is not true about the 5’-end cap structure of mature eukarytic mRNAs?)
a. The cap is methylated.
b. The cap protects the mRNA from degradation.
c. The cap is a residue of 7-methyluridine.
d. It participates in the binding of the mRNA to the ribosome to initiate translation.
15.下列何者非成熟mRNA組成?
a. Promoter
b. 5’ Cap
c. 3’ Poly A tail
d. Intron
e. Exon
16. Which of the following is not a form of RNA processed in several ways?
a. polyadenylation at 3’ end
b. proteolytic cleavage
c. splicing
d. 5’-end capping
17.利用siRNA技術可由轉錄層級使得特定基因的表達量發生何事?
某些特定基因被抑制,表達量下降
成熟的mRNA
2.何者屬於coding RNA?
mRNA
3.何者負責RNA的轉錄?
RNA聚合酶
4.RNA的序列與何者相同?
Coding strand
5.在原核細胞中,高度保留供轉錄起始識別的序列常位於哪兩個位置?
啟動子,-10、-35有高度保留
6.DNA序列上,+1代表
轉錄起始點
7.原核細胞中,何者協助RNA polymerase辨識啟動子(Promoter)?
Sigma因子
8.真核細胞中與sigma因子具有相似功能的蛋白?
TATA BOX-Binding Protein
9.TAF1於轉錄起始中的作用
#Histone乙醯化
#Histone1ubiquitination 標記降解
10.DNA上特定non-coding保留序列對基因轉錄有活化效果者稱為?
Enhancer
11.透過連結Enhancer對Transcription factor加強其作用之蛋白稱為?
Activator
12.DNA上,多個功能相關的基因由同一promoter與操控序列進行調控的特殊序列稱為?
Lac operon
13.Rho(p)因子主要參與轉錄的哪一階段
最後階段
薩諾斯啪!的一聲期末考就不見了
I am Isaac
何種序列與poly A tail 的形成有關?
AAUAAA
下列何者非成熟mRNA組成?
Promoter/5’cap/3’poly a tail/intron/exon
利用siRNA技術可由轉錄層級使得特地基因的表達量發生何事?
有些特定的基因會被抑制,表達量下降
mRNA
2.何者屬於coding RNA?
3.何者負責RNA轉錄?
RNA polymerase
RNA的序列與何者相同?
Coding srand
4.在原核細胞中,高度保留供轉錄起始識別的序列常位於那兩個位置?
-35region:TTGACA
-10region:TATAAT
5.DNA序列上,+1代表?
mRNA起點=轉錄起始點(TSS)
6.原核細胞中,何者協助RNA Polymerase辨識啟動子?
The sigma cycle
7.真核細胞中與sigma因子具有相似功能的蛋白稱為?
TATA Box-Binding protein
8.TAF1於轉錄起始中的作用為何?
乙醯化Histone
將鏈結用的H1標記以降解
9.DNA上特定non-coding保留序列對基因轉錄有活化效果者稱為?
Enhancer
透過連結Enhancer對transcription factor加強其作用之蛋白稱為?
activator
10.DNA上多個功能相關的基因由同一promotor與操控序列進行調控的特殊序列稱為?
Lac operon
11.Rho(p)因子主要參與轉錄的哪一個額階段?
結束轉錄
12.何種序列與poly A tail的形成有關?
AAUAAA
13.下列何者非成熟mRNA組成
intron
14.intron中影響mRNA splicing的的重要序列為哪些?
GU/A/AG
15.mRNA透過不同exon的組合產生不同結構蛋白的方式稱為?
Alternative spllicing
16.利用siRNA技術可由轉錄層級使得特定基因的表達量發生何事?
表達量下降
Enhancer和silencer分別為活化和抑制轉錄的"DNA保留序列"
而activator和repressor分別為加強和減弱轉錄因子的"蛋白"
關於RNA polymerase,何者為是?
1. Require a primer to initiate synthesis. RNA polymerase不需要primer
2. Require all four deoxynucleotide triphosphates(dNTPs).
3. With 3’ to 5’ exonuclease activity. mRNA的校正是一種自然發生的水解
4. Synthesis RNA in a 5’ to 3’ direction.
Ans. 4
Which of the following statements is not true about the 5’-end cap structure of mature eukaryotic mRNAs?
1. The cap is methylated.
2. The cap protects the mRNA from degradation.
3. The cap is a residue of 7-methyluridine. (7-Methylguanine)
4. It participates in the binding of the mRNA to the ribosome to initiate translation.
Ans. 2
1. Which of the following is not a form of RNA processing of eukaryotic RNA polymerase 2 primary transcrips ?
(1). Polyadenylation at 3'end
(2). Proteolytic cleavage
(沒有蛋白質的裂解)
(3). Splicing
(4). 5'-end capping
Ans:(3)
1.何者不含有Intron?
mRNA
2.何者屬於Coding RNA?
mRNA
3.何者負責RNA的轉錄? RNA的序列與何者相同?
RNA polymerase/Coding strand
4. 在原核細胞中,高度保留供轉錄起始識別的序列常位於哪兩個位置?
-10region TATAAT
-35region TTGACA
5. DNA序列上,+1代表?
mRNA的起始點也是轉錄的起始點(TSS)
6. 原核細胞中,何者協助RNA polymerase辨識啟動子(Promoter)?
σ因子
7. 真核細胞中與σ因子具有相似功能的蛋白稱為?
TATA Box-Binding Protein
8.DNA上特定non-coding保留序列對基因轉錄有活化的效果者稱為?
Enhancer
9.透過連接Enhancer對Transctiption factor加強其作用之蛋白稱為?
Activator
10. TAF1於轉錄起始中的作用為何?
乙醯化Histone
將鏈結用的H1標記以降解
11.關於RNA polymerase,何者為是?
a. require a primer to initiate synthesis.
b. require all four deoxynucleotide triphodphates (dNTPs).
c. With 3’ to 5’ exonuclease activity.
d. synthesize RNA in a 5’ to 3’ direction.
12.Rho(p)因子主要參與轉錄哪一階段?
終止RNA轉錄
13.何種序列與Poly A tail 的形成有關?
AAUAAA
14.關於真核細胞mRNA,何者錯誤?(Which of the following statements is not true about the 5’-end cap structure of mature eukarytic mRNAs?)
a. The cap is methylated.
b. The cap protects the mRNA from degradation.
c. The cap is a residue of 7-methyluridine.
d. It participates in the binding of the mRNA to the ribosome to initiate translation.
15.下列何者非成熟mRNA組成?
a. Promoter
b. 5’ Cap
c. 3’ Poly A tail
d. Intron
e. Exon
16. Which of the following is not a form of RNA processed in several ways?
a. polyadenylation at 3’ end
b. proteolytic cleavage
c. splicing
d. 5’-end capping
17.利用siRNA技術可由轉錄層級使得特定基因的表達量發生何事?
某些特定基因被抑制,表達量下降
(1). Polyadenylation at 3'end
(2). Proteolytic cleavage
(3). Splicing
(4). 5'-end capping
不會產生蛋白質的裂解—> (2)